Paper Published and Featured on Cover of EVCNA

A paper by CBM2 researchers titled, “Isolation and Analysis Methods of Extracellular Vesicles (EVs)” was published in the journal Extracellular Vesicles and Circulating Nucleic Acids in March 2021. It was also featured on the front cover of the journal. Authors of this paper include Zheng Zhao, Harshani Wijerathne, Andrew K. Godwin, and Steven A. Soper.


Extracellular vesicles (EVs) have been recognized as an evolving biomarker within the liquid biopsy family. While carrying both host cell proteins and different types of RNAs, EVs are also present in sufficient quantities in biological samples to be tested using many molecular analysis platforms to interrogate their content. However, because EVs in biological samples are comprised of both disease and non-disease related EVs, enrichment is often required to remove potential interferences from the downstream molecular assay. Most benchtop isolation/enrichment methods require > milliliter levels of sample and can cause varying degrees of damage to EVs. In addition, some of the common EV benchtop isolation methods do not sort the diseased from the non-diseased related EVs. Simultaneously, the detection of the overall concentration and size distribution of the EVs is highly dependent on techniques such as electron microscopy and Nanoparticle Tracking Analysis, which can include unexpected variations and biases as well as complexity in the analysis. This review discusses the importance of EVs as a biomarker secured from a liquid biopsy and covers some of the traditional and non-traditional, including microfluidics and resistive pulse sensing, technologies for EV isolation and detection, respectively.

You can read the entire paper at 10.20517/evcna.2021.07, and make sure to view the Author’s Talk featured alongside the article.