Two members of the CBM2 team participated in the March 17-21 PITTCON 2019 in Philadelphia, PA.

Two members of the CBM² team participated in the March 17-21 PITTCON 2019 in Philadelphia, PA.

Charuni Amarasekara, a graduate student in the Soper research group, presented her poster “Electrokinetic Separation of Single Stranded Oligonucleotides in Thermoplastic Nanochannels” on Tuesday, March 19, 2019 in the Capillary Electrophoresis poster session. Authors on the poster were Charuni Anuradha Amarasekara, Steven Allan Soper and Kumuditha M Weerakoon-Ratnayake.

Prof. Steven A. Soper presented “Nanosensors for Single-Molecule Sequencing” on Wednesday, March 20, 2019. The abstract to Dr. Soper’s talk is:

We are generating a single-molecule DNA sequencing platform that can acquire sequencing information with high accuracy. The technology employs arrays of nanosensors that read the identity of individual mononucleotides from their characteristic electrophoretic mobility and current transient amplitudes through a 2-dimensional (2D) nanochannel (~20 nm in width and depth; <10 µm in length) fabricated in a thermoplastic via nano-imprinting (NIL). The mononucleotides are generated from an intact DNA fragment using a highly processive exonuclease, which is covalently anchored to a plastic solid support contained within a bioreactor that sequentially feeds mononucleotides into the 2D nanochannel. We will discuss the operation of this nanosensor in this presentation as well as its production.